Unmodified hydrated С 60 fullerene molecules exhibit antioxidant properties, prevent damage to DNA and proteins induced by reactive oxygen species and protect mice against injuries caused by radiation-induced oxidative stress

Nanomedicine. 2019 Jan;15(1):37-46. doi: 10.1016/j.nano.2018.09.001. Epub 2018 Sep 19.

Abstract

Unmodified hydrated С60 fullerene molecules (C60UHFM) were shown to reduce the formation ROS in water and 8-oxoguanine in DNA upon ionizing radiation impact. C60UHFM efficiently eliminate long-lived protein radicals arising after irradiation. In irradiated mice C60UHFM reduce the rate of single/double-strand DNA breaks and amount of chromosomal breaks. The radioprotective activity of C60UHFM was estimated by the survival rate of animals; the dose modification factor for animal survival was 1.3. Hematological tests showed that C60UHFM injection in mice prior to irradiation results in a decrement of irradiation-induced leucopenia and thrombocytopenia. Histological analysis testified that C60UHFM provide significant protection of small intestine tissues in mice against irradiation-induced damage. The obtained data assume that the radioprotective properties of C60UHFM are determined by their antioxidant, antiradical and DNA-protective qualities. Thus, it was demonstrated that C60UHFM are a novel antioxidant and radioprotective agent capable of substantial reduction of the harmful effects of ionizing radiation.

Keywords: DNA damage; Fullerene C(60); Nanostuctures; Radioprotectors; Reactive oxygen species; X-rays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • DNA Damage*
  • Fullerenes / pharmacology*
  • Male
  • Mice
  • Mice, Hairless
  • Oxidative Stress*
  • Proteins / chemistry*
  • Radiation, Ionizing
  • Radiation-Protective Agents / pharmacology*
  • Reactive Oxygen Species / metabolism*

Substances

  • Antioxidants
  • Fullerenes
  • Proteins
  • Radiation-Protective Agents
  • Reactive Oxygen Species
  • fullerene C60