Development of Validated Methods and Quantification of Curcuminoids and Curcumin Metabolites and Their Pharmacokinetic Study of Oral Administration of Complete Natural Turmeric Formulation (Cureit™) in Human Plasma via UPLC/ESI-Q-TOF-MS Spectrometry

Molecules. 2018 Sep 20;23(10):2415. doi: 10.3390/molecules23102415.


Specific and sensitive ultra-high performance liquid chromatography-quadrupole time of flight-mass spectroscopy (UPLC-QTOF-MS) methods have been developed for the determination of curcuminoids and curcumin metabolites in human blood plasma. The UPLC-QTOF-MS method used a binary solvent delivery system and the chromatographic separation was performed on a C-18 (2.1 × 50 mm; 1.7 µm) column. Mass spectra were obtained on a Waters Xevo G2S Q-TOF mass spectrometer. The developed methods to characterize the pharmacokinetics of curcuminoids and curcumin metabolites in human blood plasma after an oral administration of bioavailable curcumin-Cureit™-were validated. It was found that the complete turmeric matrix enhances the concentration of tetrahydrocurcumin (THC), hexahydrocurcumin (HHC), octahydrocurcumin (OHC), curcumin-O-glucuronide (COG) and curcumin-O-sulfate (COS) in the blood plasma once the product is administrated.

Keywords: UPLC/ESI-Q-TOF-MS spectrometry; bioavailable curcumin (Cureit™); curcumin metabolites; curcuminoids; pharmacokinetic.

MeSH terms

  • Administration, Oral
  • Chromatography, High Pressure Liquid
  • Curcuma / chemistry*
  • Curcumin / analogs & derivatives
  • Curcumin / chemistry
  • Curcumin / pharmacokinetics
  • Curcumin / pharmacology*
  • Humans
  • Plasma / chemistry
  • Plasma / drug effects*
  • Spectrum Analysis
  • Tandem Mass Spectrometry


  • Curcumin