Methodological Issues in Studying PAHSA Biology: Masking PAHSA Effects

Cell Metab. 2018 Oct 2;28(4):543-546. doi: 10.1016/j.cmet.2018.09.007. Epub 2018 Sep 20.


PAHSAs are anti-diabetic and anti-inflammatory lipids. Syed et al. identify numerous experimental differences that likely account for the failure of Pflimlin et al. to observe PAHSA beneficial effects. The differences include different HFDs resulting in minimal/no glucose intolerance, different assay conditions, an LC-MS protocol that was not validated, and use of olive oil, a bioactive nutrient that improves glucose tolerance, as a vehicle.

Publication types

  • Letter
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Comment

MeSH terms

  • Animals
  • Blood Glucose*
  • Lipids*
  • Mice


  • Blood Glucose
  • Lipids