Antioxidant activity, neuroprotective properties and bioactive constituents analysis of varying polarity extracts from Eucalyptus globulus leaves

J Food Drug Anal. 2018 Oct;26(4):1293-1302. doi: 10.1016/j.jfda.2018.05.010. Epub 2018 Jun 18.


Eucalyptus globulus is employed as herbal tea and therapeutical purposes. In this work, it is investigated for first time the neuroprotective activities, based on antioxidant properties, of varying polarity extracts (acetone, ethanol and methanol) from E. globulus leaves and elucidate their main bioactive constituents. Methanol and acetone extracts contained the highest phenolic compounds amount and chlorogenic acid was the major compound identified by UPLC-ESI-MS/MS. Moreover, the three tested extracts showed significant antioxidant properties, varying their potency depending on the in vitro technique used. Furthermore, E.globulus extracts were effective in ameliorating H2O2-induced oxidative stress by increasing cell viability, GSH levels and antioxidant enzymes activity and, by decreasing ROS production and lipid peroxidation levels in SH-SY5Y cells. Taken together, E.globulus leaves extracts could be used as raw material for food and pharmaceutical supplements for their high content in antioxidant compounds with health benefits properties against oxidative stress.

Keywords: Antioxidants; Bioactive constituents; Eucalyptus globulus; Neuroprotection.

MeSH terms

  • Antioxidants / chemistry*
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology
  • Cell Line
  • Chlorogenic Acid / chemistry
  • Chlorogenic Acid / isolation & purification
  • Chlorogenic Acid / pharmacology
  • Eucalyptus / chemistry*
  • Glutathione / metabolism
  • Neurons / drug effects
  • Neurons / metabolism
  • Neuroprotective Agents / chemistry*
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology
  • Oxidative Stress / drug effects
  • Phenols / chemistry
  • Phenols / isolation & purification
  • Phenols / pharmacology
  • Plant Extracts / chemistry*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Plant Leaves / chemistry
  • Reactive Oxygen Species / metabolism
  • Tandem Mass Spectrometry


  • Antioxidants
  • Neuroprotective Agents
  • Phenols
  • Plant Extracts
  • Reactive Oxygen Species
  • Chlorogenic Acid
  • Glutathione