Chromosome 8 breakpoint far 3' of the c-myc oncogene in a Burkitt's lymphoma 2;8 variant translocation is equivalent to the murine pvt-1 locus

EMBO J. 1986 Nov;5(11):2845-51.

Abstract

The 2;8 variant translocation of human Burkitt's lymphomas is closely related cytogenetically to the t(6;15) of murine plasmacytomas; both involve a reciprocal exchange between the Ig kappa locus and a band region indistinguishable from that bearing the c-myc oncogene. To define their molecular relationship, we have compared cloned chromosome 8 DNA from the t(2;8) breakpoint in the human Burkitt's lymphoma JBL2 with cloned DNA from the murine pvt-1 locus, the major chromosome 15 breakpoint region in murine t(6;15). DNA sequencing and Southern blot analysis shows that these two regions are homologous. Thus the t(2;8) in JBL2 is the molecular equivalent of many murine t(6;15). The murine pvt-1 locus lies an unknown distance 3' of c-myc; analysis of DNA from several tumours with c-myc amplification reveals that pvt-1 is co-amplified in at least one case, placing pvt-1 approximately 100-500 kb 3' of c-myc. The significance of these results with respect to the role of pvt-1 in tumorigenesis is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Burkitt Lymphoma / genetics*
  • Chromosomes, Human, Pair 8*
  • DNA Restriction Enzymes
  • DNA Transposable Elements
  • Gene Amplification
  • Genes, Viral
  • Genetic Variation*
  • Humans
  • Mice
  • Oncogenes*
  • Plasmacytoma / genetics*
  • Species Specificity
  • Transcription, Genetic
  • Translocation, Genetic*

Substances

  • DNA Transposable Elements
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X04620
  • GENBANK/X04621