Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus

EMBO J. 1986 Nov;5(11):3057-63.

Abstract

A human cytomegalovirus (HCMV) glycoprotein gene with homology to glycoprotein B (gB) of herpes simplex virus and Epstein-Barr virus and gpII of varicella zoster virus has been identified by nucleotide sequencing. The gene has been expressed in recombinant vaccinia virus and the gene product recognized by monoclonal antibodies and human immune sera. Rabbits immunized with the recombinant vaccinia virus produced antibodies that immunoprecipitate gB from HCMV-infected cells and neutralize HCMV infectivity in vitro. These data demonstrate a role for this protein in future HCMV vaccines.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Antibodies, Viral / immunology*
  • Base Sequence
  • Cell Line
  • Cytomegalovirus / genetics*
  • Cytomegalovirus / immunology
  • Fluorescent Antibody Technique
  • Genes*
  • Genes, Viral*
  • Humans
  • Membrane Proteins / analysis
  • Neutralization Tests
  • Vaccinia virus / genetics*
  • Viral Envelope Proteins / genetics*
  • Viral Envelope Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Membrane Proteins
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus

Associated data

  • GENBANK/X04606