Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 80 (11), 1737-1742

Prevalence of Vibrio Parahaemolyticus in Seafood and Water Environment in the Mekong Delta, Vietnam


Prevalence of Vibrio Parahaemolyticus in Seafood and Water Environment in the Mekong Delta, Vietnam

Thi Hong To Tran et al. J Vet Med Sci.


A total of 449 samples including 385 seafood and 64 water samples in the Mekong Delta of Vietnam collected in 2015 and 2016 were examined. Of 385 seafood samples, 332 (86.2%) samples were contaminated with Vibrio parahaemolyticus and 25 (6.5%) samples were pathogenic V. parahaemolyticus carrying tdh and/or trh genes. The tdh gene positive V. parahaemolyticus strains were detected in 22 (5.7%) samples and trh gene positive V. parahaemolyticus strains were found in 5 (1.3%) samples. Of 25 pathogenic V. parahaemolyticus strains, two strains harbored both tdh and trh genes and the other 23 strains carried either tdh or trh gene. Of 64 water samples at aquaculture farms, 50 (78.1%) samples were contaminated with V. parahaemolyticus. No tdh gene positive V. parahaemolyticus strains were detected; meanwhile, trh gene positive V. parahaemolyticus strain was detected in 1 (1.6%) sample. Twenty-six pathogenic V. parahaemolyticus strains isolated were classified into 6 types of O antigen, in which the serotype O3:K6 was detected in 4 strains. All pathogenic strains were group-specific PCR negative except for 4 O3:K6 strains. The result of antimicrobial susceptibility test indicated that pathogenic strains showed high resistance rates to streptomycin (84.6%), ampicillin (57.7%) and sulfisoxazole (57.7%). These findings can be used for understanding microbiological risk of seafood in the Mekong Delta, Vietnam.

Keywords: Vibrio parahaemolyticus; antimicrobial susceptibility; serotype; tdh; trh.

Similar articles

See all similar articles


    1. Al-Othrubi S. M. Y., Kqueen C. Y., Mirhosseini H., Hadi Y. A., Radu S. 2014. Antibiotic resistance of Vibrio parahaemolyticus isolated from cockles and shrimp seafood marketed in Selangor, Malaysia. Clin. Microbiol. 3: 1–7.
    1. Bilung L. M., Radu S., Bahaman A. R., Rahim R. A., Napis S., Ling M. W. C. V., Tanil G. B., Nishibuchi M. 2005. Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by PCR. FEMS Microbiol. Lett. 252: 85–88. doi: 10.1016/j.femsle.2005.08.053 - DOI - PubMed
    1. Changchai N., Saunjit S. 2014. Occurrence of Vibrio parahaemolyticus and Vibrio vulnificus in retail raw oysters from the eastern coast of Thailand. Southeast Asian J. Trop. Med. Public Health 45: 662–669. - PubMed
    1. Chowdhury A., Ishibashi M., Thiem V. D., Tuyet D. T. N., Tung T. V., Chien B. T., Seidlein Lv L., Canh D. G., Clemens J., Trach D. D., Nishibuchi M. 2004. Emergence and serovar transition of Vibrio parahaemolyticus pandemic strains isolated during a diarrhea outbreak in Vietnam between 1997 and 1999. Microbiol. Immunol. 48: 319–327. doi: 10.1111/j.1348-0421.2004.tb03513.x - DOI - PubMed
    1. CLSI. Performance standards for antimicrobial susceptibility testing, twenty-fourth informational supplement. 2014. CLSI document M100-S24. Clinical and Laboratory Standards Institute, Wayne.