Circulating microRNAs -192 and -194 Are Associated With the Presence and Incidence of Diabetes Mellitus

Sci Rep. 2018 Sep 24;8(1):14274. doi: 10.1038/s41598-018-32274-9.

Abstract

We sought to identify circulating microRNAs as biomarkers of prevalent or incident diabetes. In a pilot study of 18 sex- and age-matched patients with metabolic syndrome, nine of whom developed diabetes during 6 years of follow-up, an array of 372 microRNAs discovered significantly elevated serum levels of microRNAs -122, -192, -194, and -215 in patients who developed diabetes mellitus type 2 (T2DM). In two cross-sectional validation studies, one encompassing sex- and age-matched groups of patients with T2DM, impaired fasting glucose (IFG) and euglycemic controls (n = 43 each) and the other 53 patients with type 1 diabetes and 54 age- and BMI-matched euglycemic controls, serum levels of miR-192, miR-194, and mi215 were significantly higher in diabetic subjects than in probands with euglycemia or IFG. In a longitudinal study of 213 initially diabetes-free patients of whom 35 developed diabetes during 6 years of follow-up, elevated serum levels of microRNAs 192 and 194 were associated with incident T2DM, independently of fasting glucose, HbA1c and other risk factors. Serum levels of miR-192 and miR-194 were also elevated in diabetic Akt2 knockout mice compared to wild type mice. In conclusion, circulating microRNAs -192 and -194 are potential biomarkers for risk of diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Animals
  • Diabetes Mellitus, Type 2 / blood*
  • Diabetes Mellitus, Type 2 / genetics
  • Diabetes Mellitus, Type 2 / pathology
  • Female
  • Humans
  • Kaplan-Meier Estimate
  • Male
  • Metabolic Syndrome / blood*
  • Metabolic Syndrome / genetics
  • Metabolic Syndrome / pathology
  • Mice
  • Mice, Knockout
  • MicroRNAs / blood*
  • Middle Aged
  • Pilot Projects
  • Prediabetic State / blood
  • Prediabetic State / genetics
  • Prediabetic State / pathology
  • Proto-Oncogene Proteins c-akt / genetics

Substances

  • MIRN122 microRNA, human
  • MIRN192 microRNA, human
  • MIRN194 microRNA, human
  • MIRN215 microRNA, human
  • MicroRNAs
  • Akt2 protein, mouse
  • Proto-Oncogene Proteins c-akt