Cyanidin-3-glucoside attenuates the angiogenesis of breast cancer via inhibiting STAT3/VEGF pathway

Phytother Res. 2019 Jan;33(1):81-89. doi: 10.1002/ptr.6201. Epub 2018 Sep 25.


Angiogenesis plays a pivotal role in breast cancer progression. Cyanidin-3-glucoside (C3G), one of the most widely distributed anthocyanins in edible fruits, shows antioxidative and anti-inflammatory property as well as induction of breast cancer cells apoptosis. However, the effect of C3G on breast cancer-induced angiogenesis remains unknown. In the present study, we found that C3G could attenuate breast cancer-induced angiogenesis via inhibiting VEGF, a key cytokine for angiogenesis, expression and secretion. Furthermore, signal transducer and activator of transcription 3 (STAT3) could transcriptionally activate VEGF, and C3G reduced STAT3 expression at both mRNA and protein level. Subsequently, our data showed that C3G induced miR-124 expression. Moreover, miR-124 could directly repress STAT3 expression, and miR-124-mediated STAT3 down-regulation was responsible for the inhibition of C3G on VEGF and angiogenesis. Taken together, we supplied more evidence to the anti-breast cancer property of C3G.

Keywords: C3G; STAT3; angiogenesis; breast cancer.

MeSH terms

  • Animals
  • Anthocyanins / metabolism*
  • Anthocyanins / pharmacology
  • Apoptosis
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Chick Embryo
  • Female
  • Glucosides / metabolism*
  • Humans
  • STAT3 Transcription Factor / metabolism*
  • Signal Transduction
  • Transfection
  • Vascular Endothelial Growth Factor A / metabolism*


  • Anthocyanins
  • Glucosides
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • Vascular Endothelial Growth Factor A
  • cyanidin-3-O-beta-glucopyranoside