Calcitonin Gene-Related Peptide Regulates the Potential Antigen Uptake Ability of Human Bronchial Epithelial Cells

J Interferon Cytokine Res. 2018 Oct;38(10):463-468. doi: 10.1089/jir.2018.0020. Epub 2018 Sep 26.

Abstract

In this study we tried to explore whether calcitonin gene-related peptide (CGRP) regulates the potential antigen uptaking ability of human bronchial epithelial cells (HBECs) and promoting the differentiation of Th1/Th2. We found that CGRP increased the uptake of fluorescein isothiocyanate labeled ovalbumin (FITC-OVA) by HBECs using fluorescence microscopy and flow cytometry analysis. MTT assay showed that T cells proliferated in a dose-dependent manner in the presence of OVA-pretreated HBECs and CGRP inhibited the proliferation of T cells. CGRP decreased secretion of IFN-γ, while it had no influence on secretion of IL-4 by ELISA. Our data suggest that CGRP enhanced HBECs antigen uptake ability and inhibits HBECs induced T cells proliferation.

Keywords: Th1/Th2differentiation; antigen uptake; calcitonin gene-related peptides; human bronchial epithelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigen Presentation / drug effects*
  • Antigen Presentation / immunology
  • Calcitonin Gene-Related Peptide / genetics
  • Calcitonin Gene-Related Peptide / immunology
  • Calcitonin Gene-Related Peptide / pharmacology*
  • Cell Differentiation / drug effects
  • Cell Differentiation / immunology
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Epithelial Cells / drug effects*
  • Epithelial Cells / immunology
  • Flow Cytometry
  • Humans
  • Microscopy, Fluorescence
  • Ovalbumin / pharmacology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology

Substances

  • Ovalbumin
  • Calcitonin Gene-Related Peptide