Binding sites of atrial natriuretic peptide in tree shrew adrenal gland

Peptides. Sep-Oct 1986;7(5):873-6. doi: 10.1016/0196-9781(86)90108-7.

Abstract

Adrenal gland binding sites for atrial natriuretic peptide-(99-126) (ANP) were quantitated in tree shrew (Tupaia belangeri) by incubation of adrenal sections with (3-[125I]-iodotyrosyl28) atrial natriuretic peptide-(99-126), followed by autoradiography with computerized microdensitometry. In the adrenal glands, there are three types of ANP binding sites. One is located in the zona glomerulosa (BMax 84 +/- 6 fmol/mg protein; Kd 122 +/- 9 pM); the second in the zona fasciculata and reticularis (BMax 29 +/- 2 fmol/mg protein; Kd 153 +/- 6 pM) and the third in the adrenal medulla (BMax 179 +/- 1 fmol/mg protein; Kd 70 +/- 2 pM). Besides the influence of ANP on the regulation of adrenocortical mineralcorticoid and glucocorticoid secretion our findings raise the possibility for a local site of action of atrial natriuretic peptide in the regulation of adrenomedullary catecholamines in the tree shrew, primates and man.

MeSH terms

  • Adrenal Glands / cytology
  • Adrenal Glands / metabolism*
  • Animals
  • Atrial Natriuretic Factor / metabolism*
  • Autoradiography
  • Iodine Radioisotopes
  • Kinetics
  • Male
  • Receptors, Atrial Natriuretic Factor
  • Receptors, Cell Surface / metabolism*
  • Tupaiidae / metabolism*

Substances

  • Iodine Radioisotopes
  • Receptors, Cell Surface
  • Atrial Natriuretic Factor
  • Receptors, Atrial Natriuretic Factor