A secretory hexokinase plays an active role in the proliferation of Nosema bombycis

PeerJ. 2018 Sep 21;6:e5658. doi: 10.7717/peerj.5658. eCollection 2018.

Abstract

The microsporidian Nosema bombycis is an obligate intracellular parasite of Bombyx mori, that lost its intact tricarboxylic acid cycle and mitochondria during evolution but retained its intact glycolysis pathway. N. bombycis hexokinase (NbHK) is not only a rate-limiting enzyme of glycolysis but also a secretory protein. Indirect immunofluorescence assays and recombinant HK overexpressed in BmN cells showed that NbHK localized in the nucleus and cytoplasm of host cell during the meront stage. When N. bombycis matured, NbHK tended to concentrate at the nuclei of host cells. Furthermore, the transcriptional profile of NbHK implied it functioned during N. bombycis' proliferation stages. A knock-down of NbHK effectively suppressed the proliferation of N. bombycis indicating that NbHK is an important protein for parasite to control its host.

Keywords: Hexokinase; Microsporidia; RNAi; Secretory protein.

Grant support

This work was supported by the National Natural Science Foundation of China (Grant number: 31472151, 31702185) and Fundamental Research Funds for the Central Universities (XDJK2015A010, XDJK2018AA001). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.