MiR-1266 promotes cell proliferation, migration and invasion in cervical cancer by targeting DAB2IP

Biochim Biophys Acta Mol Basis Dis. 2018 Dec;1864(12):3623-3630. doi: 10.1016/j.bbadis.2018.09.028. Epub 2018 Sep 24.


Cervical cancer (CC) is one of the most prevalent cancers in women in the world. However, the pathogenesis is still very unclear, and the current screening methods are too expensive. Emerging evidence shows that miR-1266 has great influence on tumor cell migration and invasion. In order to clarify the role of miR-1266 in CC, we collected serum from CC, high-grade squamous intraepithelial lesion (HSIL), low-grade squamous intraepithelial lesion (LSIL) and normal control (NC), collected tissues from CC and control group (CG), and followed up 50 CC patients. We used HeLa and SiHa cells to clarify the roles of miR-1266 on cell proliferation, migration and invasion. The CC mouse model was conducted to prove the role of miR-1266 on tumorigenesis. qRT-PCR was used to measure the expressions of miR-1266 and DAB2IP mRNA. Western blot was used to determine the expression of DAB2IP protein. Cell counting kit-8 proliferation assay (CCK-8), Colony formation assay, Wound-healing assay and Transwell invasion assay were used to determine the cell survival, proliferative, migrative and invasive abilities. Our study found that miR-1266 had a rising trend in serum from NC to LSIL to HSIL to CC, and increased in CC tissues. High expression serum miR-1266 had lower overall survival rates than patients with miR-1266 low expression. MiR-1266 promoted cell viability, proliferation, migration and invasion by targeting DAB2IP. And miR-1266 could promote tumorigenesis in vivo. In conclusion, miR-1266 could be used as a new biomarker for diagnosis, prediction and treatment of CC in the future.

Keywords: Cervical cancer; DAB2IP; Invasion; MiR-1266; Migration; Proliferation.

MeSH terms

  • Animals
  • Cell Movement
  • Cell Proliferation
  • Female
  • Gene Expression Regulation, Neoplastic*
  • HeLa Cells
  • Humans
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness / genetics*
  • Neoplasm Invasiveness / pathology
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / pathology
  • ras GTPase-Activating Proteins / genetics*


  • DAB2IP protein, human
  • MIRN1266 microRNA, human
  • MicroRNAs
  • ras GTPase-Activating Proteins