Anti-inflammatory and anti-genotoxic activity of branched chain amino acids (BCAA) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages

Jae Hoon Lee; Eunju Park; Hyue Ju Jin; Yunjeong Lee; Seung Jun Choi; Gyu Whan Lee; Pahn-Shick Chang; Hyun-Dong Paik

doi:10.1007/s10068-017-0165-4

Anti-inflammatory and anti-genotoxic activity of branched chain amino acids (BCAA) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages

Food Sci Biotechnol. 2017 Aug 18;26(5):1371-1377. doi: 10.1007/s10068-017-0165-4. eCollection 2017.

Authors

Jae Hoon Lee¹, Eunju Park², Hyue Ju Jin¹, Yunjeong Lee², Seung Jun Choi³, Gyu Whan Lee⁴, Pahn-Shick Chang⁵, Hyun-Dong Paik¹

Affiliations

¹ 1Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029 Korea.
² 2Department of Food and Nutrition, Kyungnam University, Changwon, 51767 Korea.
³ 3Department of Food Science and Technology, Department of Interdisciplinary Bio IT Materials, Seoul National University of Science and Technology, Seoul, 01811 Korea.
⁴ R&D Center, Daesang Corporation, Icheon, 17384 Korea.
⁵ 5Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826 Korea.

Abstract

The purpose of this study was to evaluate the anti-inflammatory and anti-genotoxic activity of branched-chain amino acids (BCAAs) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. BCAAs inhibited LPS-induced NO production, with 100 mM leucine having the most pronounced effect, suppressing NO production by 81.15%. Valine and isoleucine also reduced NO production by 29.65 and 42.95%, respectively. Furthermore, BCAAs suppressed the inducible nitric oxide synthase mRNA expression. Additionally, BCAAs decreased the mRNA expression of interleukin-6 and cyclooxygenase-2 which are proinflammatory mediators. Anti-genotoxic activities of BCAAs were assessed using the alkaline comet assay and valine, isoleucine, and leucine significantly (p < 0.05) decreased tail length of DNA (damaged portion) to 254.8 ± 7.5, 235.6 ± 5.6, and 271.5 ± 19.9 μm compared than positive control H₂O₂ (434.3 ± 51.3 μm). These results suggest that BCAAs can be used in the pharmaceutical or functional food industries as anti-inflammatory agents or anti-cancer agents.

Keywords: Anti-genotoxic activity; Anti-inflammatory activity; Branched-chain amino acid; RAW 264.7 macrophage.