Expression of human interleukin-2 receptor cDNA in E. coli

Biochem Biophys Res Commun. 1986 Dec 15;141(2):804-11. doi: 10.1016/s0006-291x(86)80244-3.

Abstract

cDNAs for human interleukin-2 receptor were recently cloned and sequenced (Leonard et al., 1984, Nature 311, 626-631; Nikaido et al., 1984, Nature 311, 631-635; Cosman et al., Nature 312, 768-771). In the studies reported here, we describe the expression of a cDNA clone for the human interleukin-2 receptor in E. coli using an "open reading frame" expression vector pMR100. The inserted cDNA was expressed in E. coli transformants as a tripartite fusion polypeptide fused to the lambda cI protein at its amino terminus and to beta-galactosidase at its carboxy terminus. We demonstrate that the bacterially produced IL-2 receptor protein can bind to IL-2.

MeSH terms

  • Chromosome Mapping
  • DNA / genetics
  • DNA Restriction Enzymes / metabolism
  • Escherichia coli / genetics
  • Gene Expression Regulation
  • Humans
  • Interleukin-2 / metabolism*
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / immunology
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin-2
  • Recombinant Fusion Proteins / genetics*
  • Recombinant Fusion Proteins / immunology
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Proteins / genetics*
  • Structure-Activity Relationship

Substances

  • Interleukin-2
  • Receptors, Immunologic
  • Receptors, Interleukin-2
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • DNA
  • DNA Restriction Enzymes