Muscarinic Agonist Ameliorates Insulin Secretion in Wfs1-Deficient Mice

Can J Diabetes. 2019 Mar;43(2):115-120. doi: 10.1016/j.jcjd.2018.06.007. Epub 2018 Jun 23.


Objectives: Similar to patients with Wolfram syndrome and to heterozygous Wolframin1 (Wfs1) mutation carriers, Wfs1-deficient mice exhibit impaired glucose tolerance and lower plasma insulin levels. Muscarinic receptor 3 agonists have previously been shown to potentiate glucose-stimulated insulin secretion. Therefore, the aim of this study was to investigate insulin-secretion dynamics in Wfs1-deficient mice and evaluate carbachol, muscarinic agonist and the ability to ameliorate the insulin secretion deficits caused by the Wfs1 mutation.

Methods: Wild-type Wfs1 heterozygous and Wfs1 mutant mice were used. Blood glucose was measured after glucose and carbachol administration. Insulin secretion was measured from serum using ELISA.

Results: Glucose administration causes hyperglycemia in Wfs1-deficient mice due to decreased insulin secretion. This deficit is abolished by administration of the muscarinic agonist carbachol.

Conclusions: Activation of the muscarinic pathway to potentiate insulin secretion may present a target to manage diabetes resulting from Wfs1 deficiency.

Keywords: Wolframin1 (Wfs1); diabetes; diabète; hyperglycemia; hyperglycémie; insulin secretion; muscarinic receptor M3; récepteur muscarinique de sous-type M3 (récepteur M3); sécrétion d'insuline; wolframine 1 (WFS1).

MeSH terms

  • Animals
  • Blood Glucose
  • Carbachol / pharmacology*
  • Enzyme-Linked Immunosorbent Assay
  • Glucose / pharmacology
  • Insulin Secretion / drug effects*
  • Membrane Proteins / deficiency*
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Mice
  • Mice, Knockout
  • Muscarinic Agonists / pharmacology*


  • Blood Glucose
  • Membrane Proteins
  • Muscarinic Agonists
  • wolframin protein
  • Carbachol
  • Glucose