Opioid peptides modulate production of interferon gamma by human mononuclear cells

Cell Immunol. 1986 Nov;103(1):19-26. doi: 10.1016/0008-8749(86)90064-x.


The opioid neuropeptides have previously been shown to bind to and affect leukocyte function including lymphocyte proliferation, NK-cell activity, mononuclear cell chemotaxis, immunoglobulin synthesis, and lymphokine production. The effect of the opioid peptides beta-endorphin and Met-enkephalin on interferon gamma (IFN) production by concanavalin A-stimulated human mononuclear cells was examined. Both beta-endorphin and Met-enkephalin enhanced IFN production by the majority of donor mononuclear cells tested and did so at concentrations between 10(-14) and 10(-10) M. When 10(-12) M beta-endorphin or Met-enkephalin were included in concanavalin A-stimulated mononuclear cell cultures, IFN concentrations were significantly enhanced to 205 +/- 45 and 252 +/- 67% of control, respectively. Although the majority of cell preparations tested exhibited an enhanced production of IFN in response to these opioid peptides, some did not. When beta-endorphin or Met-enkephalin were utilized at 10(-11) M, 10 of 15 and 7 of 11 responded with IFN production greater than 20% above the control (untreated) level. There was not an absolute correlation between an enhanced response to beta-endorphin and Met-enkephalin, suggesting the presence of multiple receptor types on these cells for opioids. The opioid receptor antagonist, naloxone, did not significantly prevent the opiate effect. When 10(-8) M naloxone was included in cultures containing 10(-12) M beta-endorphin or Met-enkephalin no significant inhibition of the effect of either opioid on IFN production was observed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cells, Cultured
  • Concanavalin A / pharmacology
  • Endorphins / antagonists & inhibitors
  • Endorphins / pharmacology*
  • Enkephalin, Methionine / antagonists & inhibitors
  • Enkephalin, Methionine / pharmacology*
  • Humans
  • Interferon-gamma / biosynthesis*
  • Monocytes / drug effects
  • Monocytes / metabolism*
  • Naloxone / pharmacology
  • Receptors, Opioid / classification
  • Receptors, Opioid / drug effects
  • beta-Endorphin


  • Endorphins
  • Receptors, Opioid
  • Concanavalin A
  • Naloxone
  • Enkephalin, Methionine
  • beta-Endorphin
  • Interferon-gamma