Cloning and sequence analysis of the human brain beta-adrenergic receptor. Evolutionary relationship to rodent and avian beta-receptors and porcine muscarinic receptors

FEBS Lett. 1987 Jan 26;211(2):200-6. doi: 10.1016/0014-5793(87)81436-9.

Abstract

Two cDNA clones, lambda-CLFV-108 and lambda-CLFV-119, encoding for the beta-adrenergic receptor, have been isolated from a human brain stem cDNA library. One human genomic clone, LCV-517 (20 kb), was characterized by restriction mapping and partial sequencing. The human brain beta-receptor consists of 413 amino acids with a calculated Mr of 46480. The gene contains three potential glucocorticoid receptor-binding sites. The beta-receptor expressed in human brain was homology with rodent (88%) and avian (52%) beta-receptors and with porcine muscarinic cholinergic receptors (31%), supporting our proposal [(1984) Proc. Natl. Acad. Sci. USA 81, 272 276] that adrenergic and muscarinic cholinergic receptors are structurally related. This represents the first cloning of a neurotransmitter receptor gene from human brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biological Evolution*
  • Birds / metabolism
  • Brain / metabolism*
  • Cloning, Molecular*
  • Humans
  • Infant, Newborn
  • Models, Structural
  • Receptors, Adrenergic, beta / genetics*
  • Receptors, Muscarinic / genetics
  • Rodentia / metabolism
  • Swine / metabolism

Substances

  • Receptors, Adrenergic, beta
  • Receptors, Muscarinic

Associated data

  • GENBANK/X04827