Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR)

Cold Spring Harb Protoc. 2018 Oct 1;2018(10). doi: 10.1101/pdb.prot095034.

Abstract

There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard polymerase chain reaction (PCR). In real-time PCR, it is necessary, however, to optimize the concentration of primers and probe and to perform a standard curve. It is also important to consider the data analysis method that will be used.

MeSH terms

  • Benzothiazoles
  • DNA / analysis*
  • Diamines
  • Organic Chemicals / metabolism
  • Quinolines
  • Real-Time Polymerase Chain Reaction / methods*

Substances

  • Benzothiazoles
  • Diamines
  • Organic Chemicals
  • Quinolines
  • SYBR Green I
  • DNA