Effect of blue LED on the healing process of third-degree skin burns: clinical and histological evaluation

Lasers Med Sci. 2019 Jun;34(4):721-728. doi: 10.1007/s10103-018-2647-x. Epub 2018 Oct 1.


The aim of this study was to evaluate the effects of blue light-emitting diode (LED) on the healing process of third-degree skin burns in rats through clinical and histological parameters. Forty male Wistar rats were divided into two groups: control (CTR) (n = 20) and blue LED (BLUE) (n = 20), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, and 28 days). LED (470 nm, 1 W, 12.5 J/cm2 per point, 28 s) was applied at four points of the wound (total, 50 J/cm2). Feed intake was measured every other day. It was observed that there were no statistically significant differences in the Wound Retention Index (WRI) of the BLUE group in relation to CTR group (p > 0.05) at the evaluation times. After 14, 21, and 28 days, it was observed that the animals in the BLUE group consumed more feed than animals in the CTR group (p < 0.05). At 7 days, there was a statistically significant increase in the angiogenic index (AI) in BLUE (median: 6.2) when compared to CTR (median: 2.4) (p = 0.01) and all animals in BLUE had already started re-epithelialization. This study suggests that blue LED, at the dosimetry used, positively contributed in important and initial stages of the healing process of third-degree skin burns.

Keywords: Burns; Healing; Low-intensity light therapy; Phototherapy.

MeSH terms

  • Animals
  • Burns / pathology*
  • Burns / radiotherapy*
  • Feeding Behavior
  • Granulation Tissue / pathology
  • Granulation Tissue / radiation effects
  • Inflammation / pathology
  • Light*
  • Male
  • Neovascularization, Physiologic / radiation effects
  • Phototherapy*
  • Rats, Wistar
  • Re-Epithelialization / radiation effects
  • Skin / blood supply
  • Skin / pathology*
  • Skin / radiation effects*
  • Wound Healing / radiation effects*