The class I myosin MYO1D binds to lipid and protects against colitis

Dis Model Mech. 2018 Sep 27;11(9):dmm035923. doi: 10.1242/dmm.035923.


Myosin ID (MYO1D) is a member of the class I myosin family. We screened 48,649 third generation (G3) germline mutant mice derived from N-ethyl-N-nitrosourea-mutagenized grandsires for intestinal homeostasis abnormalities after oral administration of dextran sodium sulfate (DSS). We found and validated mutations in Myo1d as a cause of increased susceptibility to DSS-induced colitis. MYO1D is produced in the intestinal epithelium, and the colitis phenotype is dependent on the nonhematopoietic compartment of the mouse. Moreover, MYO1D appears to couple cytoskeletal elements to lipid in an ATP-dependent manner. These findings demonstrate that MYO1D is needed to maintain epithelial integrity and protect against DSS-induced colitis.

Keywords: Dextran sodium sulfate; Inflammatory bowel disease; N-ethyl-N-nitrosourea.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / metabolism
  • Animals
  • Cell Differentiation
  • Colitis / genetics
  • Colitis / metabolism*
  • Colitis / pathology
  • Colitis / prevention & control*
  • Dextran Sulfate
  • Epithelial Cells / metabolism
  • Epithelial Cells / pathology
  • Female
  • Genes, Recessive
  • Hematopoiesis
  • Intestinal Mucosa / pathology
  • Intestines / pathology
  • Membrane Lipids / metabolism*
  • Mice, Inbred C57BL
  • Mutation / genetics
  • Myosins / metabolism*
  • Phenotype
  • Reproducibility of Results


  • Membrane Lipids
  • Dextran Sulfate
  • Myo1d protein, mouse
  • Myosins