Anti-inflammatory effects of Kaempferol on Helicobacter pylori-induced inflammation

Biosci Biotechnol Biochem. 2019 Jan;83(1):166-173. doi: 10.1080/09168451.2018.1528140. Epub 2018 Oct 5.


Inflammation induced by Helicobacter pylori infection related to gastric carcinogenesis. In this study, we have investigated the anti-inflammatory effect and its mechanism of kaempferol in the inflammatory response caused by H. pylori infection in vitro. We found that kaempferol reduced the expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-8) and production of IL-8 in AGS cells. In addition, kaempferol suppressed translocation of cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA) of H. pylori to AGS cells. It was due to decreased transcription of type IV secretion system (T4SS) components involved in CagA injection and secretion system subunit protein A (SecA) of type V secretion system (T5SS) involved in VacA secretion by kaempferol. In conclusion, kaempferol shows the anti-inflammatory effect by suppressing the translocation of CagA and VacA proteins and leading to the down-regulation of pro-inflammatory cytokines. Abbreviations: CagA: cytotoxin-associated gene A; VacA: vacuolating cytotoxin A; T4SS: type IV secretion systems; SecA: secretion system subunit protein A; T5SS: type V secretion system.

Keywords: CagA; Helicobacter pylori; VacA; kaempferol; pro-inflammatory cytokines.

MeSH terms

  • Anti-Inflammatory Agents / pharmacology*
  • Antigens, Bacterial / drug effects
  • Antigens, Bacterial / metabolism
  • Bacterial Proteins / drug effects
  • Bacterial Proteins / metabolism
  • Gastritis / microbiology*
  • Gastritis / prevention & control*
  • Helicobacter Infections / complications
  • Helicobacter Infections / microbiology
  • Helicobacter Infections / prevention & control*
  • Helicobacter pylori / drug effects*
  • Helicobacter pylori / growth & development
  • Helicobacter pylori / pathogenicity*
  • Humans
  • Inflammation / etiology
  • Inflammation / prevention & control*
  • Inflammation Mediators / metabolism
  • Interleukin-1beta / metabolism
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / metabolism
  • Kaempferols / pharmacology*
  • Protein Transport / drug effects
  • Transforming Growth Factor alpha / metabolism


  • Anti-Inflammatory Agents
  • Antigens, Bacterial
  • Bacterial Proteins
  • CXCL8 protein, human
  • IL1B protein, human
  • Inflammation Mediators
  • Interleukin-1beta
  • Interleukin-8
  • Kaempferols
  • TGFA protein, human
  • Transforming Growth Factor alpha
  • VacA protein, Helicobacter pylori
  • cagA protein, Helicobacter pylori
  • kaempferol