In Vitro Hydrodynamic Evaluation of a Scaffold for Heart Valve Tissue Engineering

Artif Organs. 2019 Feb;43(2):195-198. doi: 10.1111/aor.13293. Epub 2018 Oct 5.

Abstract

Although prosthetic heart valves have saved many lives, the search for a living substitute continues with the aid of tissue engineering. Much progress has been made so far, but the translation of this technology to clinical reality remains a challenge, especially due to the structural complexity of heart valves and the harsh environment they are in. In a joint effort, researchers from Federal University of ABC and Institute Dante Pazzanese of Cardiology have conceived a new bioresorbable scaffold for heart valve tissue engineering (HVTE), whose hydrodynamic performance was first assessed and described in this work. The scaffold was studied at the mitral position of a left heart simulator from Escola Politécnica of the University of São Paulo, under 60 bpm and with no cell seeding. In this condition, two-dimensional particle image velocimetry was performed to investigate the flow during diastolic and systolic phases. The results indicate that the scaffold can withstand the required intraventricular pressures for a simulated normal physiologic condition in a bioreactor. Furthermore, the averaged (N = 150) velocity vector maps showed a smooth and well-distributed flow during diastole and qualitatively demonstrated no-significant regurgitation at systole.

Keywords: Heart valve tissue engineering; Left ventricular model; Scaffold evaluation; Two-dimensional particle image velocimetry.

MeSH terms

  • Heart Valve Prosthesis*
  • Humans
  • Hydrodynamics*
  • Tissue Engineering*
  • Tissue Scaffolds*