Lucigenin chemiluminescence in the assessment of neutrophil superoxide production

J Immunol Methods. 1987 Mar 12;97(2):209-13. doi: 10.1016/0022-1759(87)90461-3.

Abstract

Lucigenin-amplified chemiluminescence (LUCL) was induced by .O2- (from the xanthine/xanthine-oxidase reaction) but not, as with luminol-augmented CL (LCL), by myeloperoxidase (MPO) and only weakly by H2O2 or the H2O2-MPO-Cl- system. Neutrophil LUCL induced by fMet-Leu-Phe (fMLP) was dose-dependently inhibited by scavengers of .O2- but not by NaN3, catalase, mannitol or taurine. Response patterns of several soluble stimuli (leukotriene B4 (LTB4), platelet-activating factor (PAF), fMLP, A23187 and phorbol-myristate-acetate (PMA)) were assessed with LUCL. PMA-induced LUCL was protracted, A23187 showed intermediary kinetics whereas fMLP and PAF both showed rapid peak responses at 30-50 s. However, LTB4 was the most rapid initiator of LUCL (7-12 s). The kinetics of fMLP- or PMA-induced neutrophil .O2- production, assessed with the cytochrome C reduction method, correlated well with LUCL. Thus it is suggested that LUCL provides a specific method for studying the kinetic properties of neutrophil .O2- production where stimulus-specific response patterns can be distinguished.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acridines*
  • Free Radicals
  • Humans
  • Leukotriene B4 / pharmacology
  • Luminescent Measurements
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / metabolism*
  • Peroxidase / pharmacology
  • Superoxides / metabolism*

Substances

  • Acridines
  • Free Radicals
  • Superoxides
  • Leukotriene B4
  • 10,10'-dimethyl-9,9'-biacridinium
  • N-Formylmethionine Leucyl-Phenylalanine
  • Peroxidase