Chemiluminescence-based biosensor for monitoring astronauts' health status during space missions: Results from the International Space Station

Biosens Bioelectron. 2019 Mar 15;129:260-268. doi: 10.1016/j.bios.2018.09.059. Epub 2018 Sep 17.


During space missions, real-time monitoring of astronauts' health status is of crucial importance and therefore there is a strong demand for simple analytical devices that astronauts can use to perform clinical chemistry analyses directly onboard. As part of the "IN SITU Bioanalysis" project, we designed a biosensor for analysing salivary levels of cortisol in astronauts, a marker of chronic stress. The biosensor is based on the Lateral Flow Immunoassay (LFIA) approach coupled with chemiluminescence (CL) detection and comprises a 3D-printed plastic cartridge containing a sealed fluidic element with the LFIA strip, in which the flow of sample and reagents is activated by pressing buttons on the cartridge and sustained by exploiting capillary forces. For measurement, the photon emission is imaged employing a CL reader based on an ultrasensitive cooled charge-coupled device (CCD) camera. The payload was designed to operate in microgravity and to withstand mechanical stress, such as take-off vibrations, and onboard depressurization events, while the microfluidics was developed considering alterations of physical phenomena occurring in microgravity, such as bubble formation, surface wettability and liquid evaporation. The biosensor, which was successfully used by the Italian astronaut Paolo Nespoli during the VITA mission (July-December 2017), demonstrated the feasibility of performing sensitive LFIA analysis of salivary cortisol down to 0.4 ng/mL directly onboard the International Space Station. It could be easily adapted for the analysis of other clinical biomarkers, thus enabling the early diagnosis of diseases and the timely activation of appropriate countermeasures.

Keywords: Chemiluminescence biosensor; Cortisol; International Space Station; Lateral-flow immunoassay; Point-of-care testing; Space medicine.

Publication types

  • Evaluation Study

MeSH terms

  • Antibodies, Immobilized / chemistry
  • Astronauts
  • Biosensing Techniques / instrumentation*
  • Equipment Design
  • Health Status
  • Humans
  • Hydrocortisone / analysis*
  • Immunoenzyme Techniques / instrumentation
  • Limit of Detection
  • Luminescent Measurements / instrumentation*
  • Printing, Three-Dimensional
  • Reagent Strips / analysis*
  • Saliva / chemistry*
  • Space Flight
  • Weightlessness


  • Antibodies, Immobilized
  • Reagent Strips
  • Hydrocortisone