Specific discrimination of gram-positive bacteria and direct visualization of its infection towards mammalian cells by a DPAN-based AIEgen

Rong Hu; Fan Zhou; Taotao Zhou; Jianlei Shen; Zhiming Wang; Zujin Zhao; Anjun Qin; Ben Zhong Tang

doi:10.1016/j.biomaterials.2018.09.019

Specific discrimination of gram-positive bacteria and direct visualization of its infection towards mammalian cells by a DPAN-based AIEgen

Biomaterials. 2018 Dec:187:47-54. doi: 10.1016/j.biomaterials.2018.09.019. Epub 2018 Sep 28.

Authors

Rong Hu¹, Fan Zhou¹, Taotao Zhou¹, Jianlei Shen¹, Zhiming Wang², Zujin Zhao¹, Anjun Qin³, Ben Zhong Tang⁴

Affiliations

¹ State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-induced Emission, Guangzhou International Campus, South China University of Technology, Guangzhou, 510640, China.
² State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-induced Emission, Guangzhou International Campus, South China University of Technology, Guangzhou, 510640, China; HKUST-Shenzhen Research Institute, Shenzhen, 518057, China. Electronic address: wangzhiming@scut.edu.cn.
³ State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-induced Emission, Guangzhou International Campus, South China University of Technology, Guangzhou, 510640, China. Electronic address: msqinaj@scut.edu.cn.
⁴ State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-induced Emission, Guangzhou International Campus, South China University of Technology, Guangzhou, 510640, China; HKUST-Shenzhen Research Institute, Shenzhen, 518057, China; Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Institute for Advanced Study, and Department of Chemical and Biological Engineering, The Hong Kong University of Science & Technology (HKUST), Clear Water Bay, Kowloon, Hong Kong, China. Electronic address: tangbenz@ust.hk.

PMID: 30292941
DOI: 10.1016/j.biomaterials.2018.09.019

Abstract

The infections caused by gram-positive bacteria (G⁺) is a major threat to public health due to their high morbidity and mortality, and the identification of G⁺ with high sensitivity and selectivity is urgently desirable. In this paper, a probe with aggregation-induced emission (AIE) characteristic is developed and used to discriminate G⁺ from other bacteria and fungus with excellent selectivity. Thanks for its AIE feature, this probe shows intense fluorescence when interacted with G⁺, and the signal can last for 24 h after incubating with S. aureus, which is benefit for long term tracing of translocation process with high contrast. Moreover, this probe can be used to visualize the infection processes of G⁺ towards mammalian cells, which provides valuable information for understanding the therapeutic mechanism.

Keywords: Aggregation-induced emission; Discrimination; Gram-positive bacteria; Specificity; Visualization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Adsorption
Animals
Cell Line
Fluorescent Dyes / chemistry*
Gram-Positive Bacteria / isolation & purification*
Gram-Positive Bacterial Infections / microbiology*
HeLa Cells
Humans
Mice
Morpholines / chemistry*
Naphthalenes / chemistry*
Optical Imaging
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Morpholines
Naphthalenes