Complete nucleotide sequence of the topA gene encoding Escherichia coli DNA topoisomerase I

J Mol Biol. 1986 Oct 5;191(3):321-31. doi: 10.1016/0022-2836(86)90129-4.

Abstract

The nucleotide sequence of a 4071 base-pair long segment containing the gene topA encoding Escherichia coli DNA topoisomerase I and its flanking regions has been determined. The gene encodes a total of 864 amino acids from the ATG start to a TAA termination codon, of which the first f-Met appears to be removed after translation; the calculated molecular weight of the translated protein is 97,413. Mapping of promoters by deletion of sequences upstream from the ATG initiation codon indicates the existence of at least two promoters that direct transcription into topA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Base Sequence
  • Codon
  • DNA Topoisomerases, Type I / genetics*
  • DNA, Bacterial / genetics*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Promoter Regions, Genetic

Substances

  • Amino Acids
  • Codon
  • DNA, Bacterial
  • DNA Topoisomerases, Type I

Associated data

  • GENBANK/X04475