Background: The biomimetic self-assembling peptide technology is a paradigm for dental hard tissue regeneration.
Aim: To investigate the efficacy of biomimetic self-assembling peptide (P11-4) on enamel remineralization compared to casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) and fluoride-based delivery systems.
Materials and methods: Artificial enamel lesions were created on buccal surfaces of 40 extracted human molars. Specimens were randomly assigned to four groups (n = 10) according to the remineralizing agent used: G1 - control: artificial saliva, G2 - fluoride varnish, G3 - CPP-ACPF varnish, G4 - self-assembling peptide agent. All products were applied according to the manufacturer's instructions and the specimens were stored in daily renewed artificial saliva. Surface microhardness (SMH) was assessed at baseline, after demineralization, after 1 week and after 4 weeks storage. SMH values were analyzed using ANOVA and Tukey's post hoc test.
Results: Self-assembling peptide showed the highest statistically significant mean SMH followed by fluoride and CPP-ACPF while the lowest mean SMH was found in artificial saliva. However, no statistically significant difference was found between fluoride and CPP-ACPF. Higher statistically significant mean SMH was found after 4 weeks compared to 1 week remineralization in all groups.
Conclusions: Self-assembling peptide confers the highest remineralizing efficacy compared to fluoride and CPP-ACPF, showing a promising, noninvasive regeneration potential. Furthermore, extended period of time helped attain more benefits from the remineralizing regimens applied.
Keywords: Biomimetic remineralization; enamel regeneration; self-assembling peptides; surface microhardness.
Conflict of interest statement
There are no conflicts of interest.
Complementary remineralizing effect of self-assembling peptide (P11-4) with CPP-ACPF or fluoride: An in vitro study.J Clin Exp Dent. 2020 Feb 1;12(2):e161-e168. doi: 10.4317/jced.56295. eCollection 2020 Feb. J Clin Exp Dent. 2020. PMID: 32071698 Free PMC article.
Remineralizing efficacy of different calcium-phosphate and fluoride based delivery vehicles on artificial caries like enamel lesions.J Dent. 2014 Apr;42(4):466-74. doi: 10.1016/j.jdent.2013.12.017. Epub 2014 Jan 9. J Dent. 2014. PMID: 24412586
Analysis of efficacy of the self-assembling peptide-based remineralization agent on artificial enamel lesions.Microsc Res Tech. 2019 Jul;82(7):1065-1072. doi: 10.1002/jemt.23254. Epub 2019 Mar 18. Microsc Res Tech. 2019. PMID: 30884045
An in vitro comparison of casein phosphopeptide-amorphous calcium phosphate paste, casein phosphopeptide-amorphous calcium phosphate paste with fluoride and casein phosphopeptide-amorphous calcium phosphate varnish on the inhibition of demineralization and promotion of remineralization of enamel.J Indian Soc Pedod Prev Dent. 2017 Oct-Dec;35(4):312-318. doi: 10.4103/JISPPD.JISPPD_308_16. J Indian Soc Pedod Prev Dent. 2017. PMID: 28914243
Treatment of Carious Lesions Using Self-Assembling Peptides.Adv Dent Res. 2018 Feb;29(1):42-47. doi: 10.1177/0022034517737025. Adv Dent Res. 2018. PMID: 29355413 Review.
- Pretty IA, Ellwood RP. The caries continuum: Opportunities to detect, treat and monitor the re-mineralization of early caries lesions. J Dent. 2013;41(Suppl 2):S12–21. - PubMed
- Featherstone JD. Dental caries: A dynamic disease process. Aust Dent J. 2008;53:286–91. - PubMed
- Goswami M, Saha S, Chaitra TR. Latest developments in non-fluoridated remineralizing technologies. J Indian Soc Pedod Prev Dent. 2012;30:2–6. - PubMed
- Amaechi BT. Remineralization therapies for initial caries lesions. Curr Oral Health Rep. 2015;2:95–101.
- Kirkham J, Firth A, Vernals D, Boden N, Robinson C, Shore RC, et al. Self-assembling peptide scaffolds promote enamel remineralization. J Dent Res. 2007;86:426–30. - PubMed