Gene disruption indicates that the only essential function of the SKI8 chromosomal gene is to protect Saccharomyces cerevisiae from viral cytopathology

Virology. 1987 Mar;157(1):252-6. doi: 10.1016/0042-6822(87)90338-2.

Abstract

We have cloned the SKI8 gene, one of the chromosomal genes that repress replication of M, L-A, and L-BC double-stranded RNA viruslike particles in yeast. The clone was used to map SKI8 to chromosome VII near ade5 and to construct a deletion mutant. The deletion mutant was unable to grow at 8 degrees if and only if M1 double-stranded RNA was present.

MeSH terms

  • Chromosome Mapping
  • Chromosomes / physiology
  • Cloning, Molecular
  • DNA Replication*
  • DNA Restriction Enzymes
  • Genes, Viral*
  • Plasmids
  • Saccharomyces cerevisiae / genetics*
  • Virus Replication
  • Viruses / genetics*

Substances

  • DNA Restriction Enzymes