Gene Sequence Screening for Manganese Poisoning-Susceptible Genes and Analysis of Gene Interaction Effects

Environ Toxicol Pharmacol. 2018 Dec;64:60-69. doi: 10.1016/j.etap.2018.09.013. Epub 2018 Sep 29.

Abstract

Manganese poisoning is a common occupational disease, studies have found that the susceptibility to manganese poisoning differs in individuals. We adopted genome-wide sequencing methods to screen for susceptibility genes involved in gene-mediated metabolic pathways from the perspective of manganese poisoning. We identified 18,439 genes in this study, including 14,272 known genes and 4398 new genes. We then selected 17 differential genes using p values, of which 7 genes were down-regulated and 10 genes were up-regulated. Possible interaction genes for each differential gene were selected according to the String database. Sgk1, HCRTr1, HspB1, Rem2, Oprd1, ATF5, and TRHr identified in this study may be involved in oxidative stress mechanisms, dopamine (DA) synthesis, and neuronal survival during apoptosis and may affect susceptibility to manganese poisoning.

Keywords: Gene analysis; Interaction genes; Manganese poisoning; Susceptibility genes.

MeSH terms

  • Animals
  • Corpus Striatum / metabolism*
  • Epistasis, Genetic
  • Genetic Predisposition to Disease*
  • Male
  • Manganese Poisoning / genetics*
  • Rats, Sprague-Dawley
  • Transcriptome