The disparate effects of bacteriophages on antibiotic-resistant bacteria

Abstract

Faced with the crisis of multidrug-resistant bacteria, bacteriophages, viruses that infect and replicate within bacteria, have been reported to have both beneficial and detrimental effects with respect to disease management. Bacteriophages (phages) have important ecological and evolutionary impacts on their bacterial hosts and have been associated with therapeutic use to kill bacterial pathogens, but can lead to the transmission of antibiotic resistance. Although the process known as transduction has been reported for many bacterial species by classic and modern genetic approaches, its contribution to the spread of antibiotic resistance in nature remains unclear. In addition, detailed molecular studies have identified phages residing in bacterial genomes, revealing unexpected interactions between phages and their bacterial hosts. Importantly, antibiotics can induce the production of phages and phage-encoded products, disseminating these viruses and virulence-related genes, which have dangerous consequences for disease severity. These unwanted side-effects of antibiotics cast doubt on the suitability of some antimicrobial treatments and may require new strategies to prevent and limit the selection for virulence. Foremost among these treatments is phage therapy, which could be used to treat many bacterial infectious diseases and confront the pressing problem of antibiotic resistance in pathogenic bacteria. This review discusses the interactions between bacteriophages, antibiotics, and bacteria and provides an integrated perspective that aims to inspire the development of successful antibacterial therapies.

Fig. 1. Infection and injection of DNA carried by phages or transducing particles into a bacterial cell.

Different processes affecting bacteria can be induced afterwards such as: a lysis (by virulent phages), b lysogeny (by temperate phages) and c DNA recombination/transduction (by transducing particles)

Fig. 2. Relative effect of temperate, virulent phages and antibiotics (colored circles) on different bacterial traits as represented by the position of the circles (hatched circles).

For example, although antibiotics are primarily responsible for antibiotic resistance, temperate phages also play a role