Drug metabolism by the human hepatoma cell, Hep G2

Biochem Biophys Res Commun. 1987 Feb 27;143(1):52-7. doi: 10.1016/0006-291x(87)90628-0.


The human liver-derived cell line, Hep G2, has aryl hydrocarbon hydroxylase and 7-ethoxycoumarin o-de-ethylase activities. Partial purification of cytochrome P-450 from Hep G2 cells provided spectral evidence of this hemeprotein in the purified fraction. These results suggest that Hep G2 cells will be useful for the study of cytochrome P-450 and the regulation of mixed function oxidase activities in liver cells of human origin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 7-Alkoxycoumarin O-Dealkylase
  • Aryl Hydrocarbon Hydroxylases / metabolism*
  • Carcinoma, Hepatocellular / metabolism*
  • Cell Line
  • Cytochrome P-450 Enzyme System / isolation & purification
  • Cytochrome P-450 Enzyme System / metabolism*
  • Humans
  • Kinetics
  • Liver Neoplasms / metabolism*
  • Microsomes / metabolism
  • Oxygenases / metabolism*


  • Cytochrome P-450 Enzyme System
  • Oxygenases
  • 7-Alkoxycoumarin O-Dealkylase
  • Aryl Hydrocarbon Hydroxylases