Oxidation-reduction midpoint potentials of the molybdenum center in spinach NADH:nitrate reductase

FEBS Lett. 1987 Mar 23;213(2):372-4. doi: 10.1016/0014-5793(87)81524-7.

Abstract

Oxidation-reduction midpoint potentials for the molybdenum center in assimilatory NADH:nitrate reductase isolated from spinach (Spinacia oleracea) have been determined at pH 7.0 in the presence of dye mediators using EPR spectroscopy to monitor formation of Mo(V). Values for the Mo(VI)/Mo(V) and Mo(V)/Mo(IV) couples were determined to be -8 and -42 mV, respectively.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chlorella / enzymology
  • Electron Spin Resonance Spectroscopy
  • Hydrogen-Ion Concentration
  • Molybdenum / metabolism*
  • Nitrate Reductase (NADH)
  • Nitrate Reductases / metabolism*
  • Oxidation-Reduction
  • Plants / enzymology*
  • Vegetables

Substances

  • Molybdenum
  • Nitrate Reductases
  • Nitrate Reductase (NADH)