Interaction between the DNA polymerase and single-stranded DNA-binding protein (infected cell protein 8) of herpes simplex virus 1

J Biol Chem. 1987 Mar 25;262(9):4260-6.


The herpes virus-encoded DNA replication protein, infected cell protein 8 (ICP8), binds specifically to single-stranded DNA with a stoichiometry of one ICP8 molecule/12 nucleotides. In the absence of single-stranded DNA, it assembles into long filamentous structures. Binding of ICP8 inhibits DNA synthesis by the herpes-induced DNA polymerase on singly primed single-stranded DNA circles. In contrast, ICP8 greatly stimulates replication of circular duplex DNA by the polymerase. Stimulation occurs only in the presence of a nuclear extract from herpes-infected cells. Appearance of the stimulatory activity in nuclear extracts coincides closely with the time of appearance of herpes-induced DNA replication proteins including ICP8 and DNA polymerase. A viral factor(s) may therefore be required to mediate ICP8 function in DNA replication.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriophages / genetics
  • DNA Replication
  • DNA, Circular / metabolism
  • DNA, Single-Stranded / metabolism
  • DNA, Viral / metabolism
  • DNA-Binding Proteins
  • DNA-Directed DNA Polymerase / metabolism*
  • Macromolecular Substances
  • Microscopy, Electron
  • Nucleic Acid Synthesis Inhibitors
  • Protein Conformation
  • Simplexvirus / enzymology
  • Viral Proteins / metabolism*
  • Viral Proteins / pharmacology


  • DNA, Circular
  • DNA, Single-Stranded
  • DNA, Viral
  • DNA-Binding Proteins
  • ICP8 protein, Simplexvirus
  • Macromolecular Substances
  • Nucleic Acid Synthesis Inhibitors
  • Viral Proteins
  • DNA-Directed DNA Polymerase