Molecular cloning of a cDNA for a human ADP/ATP carrier which is growth-regulated

J Biol Chem. 1987 Mar 25;262(9):4355-9.


We have identified in a human cDNA library a clone (hp2F1) whose cognate RNA is growth-regulated. The insert has been sequenced and the nucleotide sequence shows a strong homology to the nucleotide sequences of the ADP/ATP carrier cDNA and gene, respectively, isolated from Neurospora crassa and Saccharomyces cerevisiae. The putative amino acid sequence of hp2F1 shows an 87% homology to the amino acid sequence of the ADP/ATP carrier from beef heart mitochondria. We conclude that the insert of hp2F1 contains the full coding sequence of a human ADP/ATP carrier. The steady-state RNA levels of the ADP/ATP carrier are growth-regulated. They increase when quiescent cells are stimulated by serum, platelet-derived growth factor, or epidermal growth factor, but not by platelet-poor plasma or insulin. RNA levels of the ADP/ATP carrier decrease instead when growing HL-60 cells are induced to differentiate by either phorbol esters or retinoic acid.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blood
  • Cattle
  • Cell Differentiation
  • Cell Line
  • Cloning, Molecular*
  • DNA / genetics*
  • Epidermal Growth Factor / pharmacology
  • Growth Substances / pharmacology*
  • Humans
  • Leukemia, Myeloid / genetics
  • Leukemia, Myeloid / pathology
  • Mice
  • Mitochondria, Heart / enzymology
  • Mitochondrial ADP, ATP Translocases / genetics*
  • Neurospora crassa / genetics
  • Nucleotidyltransferases / genetics*
  • Platelet-Derived Growth Factor / pharmacology
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / genetics


  • Growth Substances
  • Platelet-Derived Growth Factor
  • RNA, Messenger
  • Epidermal Growth Factor
  • DNA
  • Mitochondrial ADP, ATP Translocases
  • Nucleotidyltransferases

Associated data

  • GENBANK/J02683