Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5

J Cell Physiol. 1987 Mar;130(3):420-7. doi: 10.1002/jcp.1041300316.


The SV40 transformed murine macrophage cell line, BAC1, proliferates in response to the colony stimulating factor, CSF-1 (Schwarzbaum et al., J. Immunol., 132:1158, 1984). In order to obtain a cell line suitable for biochemical and genetic studies of CSF-1 signal transduction, clones of BAC1 were established. Clones ranged from being completely autonomous to being completely dependent on CSF-1 for growth. Cells of one clone (2F5), which proliferated in response to either CSF-1 or granulocyte-macrophage CSF (GM-CSF) were characterized in detail. The kinetics of receptor-mediated internalization and intracellular destruction of CSF-1 were comparable to the kinetics observed with peritoneal exudate macrophages. CSF-1 was shown to regulate cell spreading, cell survival, protein degradation, and the duration of the G1 and S phases of the cell cycle. The 2F5 clone therefore exhibits a number of CSF-1 stimulated responses and is being used for genetic and biochemical studies of CSF-1 action.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Division
  • Cell Line
  • Cell Survival
  • Clone Cells
  • Colony-Stimulating Factors / pharmacology*
  • Macrophages / cytology*
  • Macrophages / drug effects
  • Mice
  • Protein Biosynthesis
  • Proteins / metabolism
  • Simian virus 40 / genetics
  • Temperature


  • Colony-Stimulating Factors
  • Proteins