Dual cycle amplification and dual signal enhancement assisted sensitive SERS assay of MicroRNA

Yingdi Wu; Ying Li; Huixia Han; Caisheng Zhao; Xiaoru Zhang

doi:10.1016/j.ab.2018.10.004

Dual cycle amplification and dual signal enhancement assisted sensitive SERS assay of MicroRNA

Anal Biochem. 2019 Jan 1:564-565:16-20. doi: 10.1016/j.ab.2018.10.004. Epub 2018 Oct 9.

Authors

Yingdi Wu¹, Ying Li¹, Huixia Han², Caisheng Zhao², Xiaoru Zhang³

Affiliations

¹ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
² Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
³ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China. Electronic address: zhangxr7407@126.com.

PMID: 30312618
DOI: 10.1016/j.ab.2018.10.004

Abstract

A sensitive surface-enhanced Raman scattering (SERS) approach has been developed for detection of microRNA (miRNA) based on target-triggered dual signal amplification including strand displancement amplification (SDA) and hybridization chain reaction (HCR). With the assistant of polymerase and nicking endonuclease (NEase), target miRNA combines with the single stranded template DNA to generate a great amount of trigger DNA which can induce HCR. Coupled the dual cycle amplification of SDA and HCR with the dual enhancement of gold nanoparticles (AuNPs), a low detection limit of 0.5 fM for miRNA is obtained using the proposed strategy. With high sensitivity, universality, rapid analysis, and high selectivity, this method has a great potential for detecting biomolecules with trace amounts in bioanalysis and clinical biomedicine.

Keywords: Dual cycle amplification; Dual signal enhancement; Surface-enhanced Raman scattering; miRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gold / chemistry
Limit of Detection
Metal Nanoparticles / chemistry
MicroRNAs / analysis*
Spectrum Analysis, Raman / methods*

Substances

MicroRNAs
Gold