Synthetic biology tools for engineering Yarrowia lipolytica

Abstract

The non-conventional oleaginous yeast Yarrowia lipolytica shows great industrial promise. It naturally produces certain compounds of interest but can also artificially generate non-native metabolites, thanks to an engineering process made possible by the significant expansion of a dedicated genetic toolbox. In this review, we present recently developed synthetic biology tools that facilitate the manipulation of Y. lipolytica, including 1) DNA assembly techniques, 2) DNA parts for constructing expression cassettes, 3) genome-editing techniques, and 4) computational tools.

Keywords: CRISPR-Cas9; DNA assembly; Genome editing; Genome scale metabolic models; Golden Gate; Industrial biotechnology; Metabolic engineering; Synthetic biology; Yarrowia lipolytica.