Zinc deficiency drives Th17 polarization and promotes loss of Treg cell function

J Nutr Biochem. 2019 Jan:63:11-18. doi: 10.1016/j.jnutbio.2018.09.011. Epub 2018 Sep 21.

Abstract

A high number of illnesses and disorders are connected to zinc deficiency. Equally, T cell polarization and a balance between different T helper (Th) cell subsets are essential. Therefore, in this study, the influence of zinc deficiency on T cell polarization and on respective signaling pathways was investigated. We uncovered a significantly increased number of regulatory T cells (Treg) and Th17 cells in expanded T cells during zinc deficiency after 3 days of combined treatment with IL-2 and TGF-β1 (Treg) or IL-6 and TGF-β1 (Th17). No difference in Th1 and Th2 cell polarization between zinc-deficient and zinc-adequate status was prominent. On the molecular level, Smad signaling was significantly enhanced by stimulation with TGF-β1/IL-6 during zinc deficiency compared to adequate zinc condition. This represents an explanation for the elevated Th17 cell numbers associated with autoimmune disease especially during zinc deficiency. Moreover, Treg cell numbers are increased during zinc deficiency as well. However, those cells might be nonfunctional since a lower expression of miR-146a was uncovered compared to normal zinc concentrations. In summary, an adequate zinc homeostasis is fundamental to slow down or probably stop the progression of autoimmune diseases and infections. Therefore, supplementing zinc might be a therapeutic approach to dampen autoimmune diseases connected to Th17 cells.

Keywords: Normal zinc concentration; T helper cell; T helper cell polarization; Th cell; ZD; ZN; Zinc deficiency.

MeSH terms

  • Cell Polarity
  • Cells, Cultured
  • Gene Expression Regulation
  • Humans
  • Jurkat Cells
  • Leukocytes, Mononuclear
  • MicroRNAs / genetics
  • Proteins / metabolism
  • T-Lymphocytes, Regulatory / immunology
  • T-Lymphocytes, Regulatory / physiology*
  • Th17 Cells / immunology
  • Th17 Cells / pathology*
  • Zinc / deficiency*
  • Zinc / metabolism

Substances

  • MIRN146 microRNA, human
  • MicroRNAs
  • Proteins
  • Zinc