Spindlin1 alters the metaphase to anaphase transition in meiosis I through regulation of BUB3 expression in porcine oocytes

J Cell Physiol. 2019 Jun;234(6):8963-8974. doi: 10.1002/jcp.27566. Epub 2018 Oct 14.


Spindlin 1 (SPIN1), which contains Tudor-like domains, regulates maternal transcripts via interaction with a messenger RNA (mRNA)-binding protein. SPIN1 is involved in tumorigenesis in somatic cells and is highly expressed in cancer cells. Nevertheless, the role of SPIN1 in porcine oocyte maturation remains totally unknown. To explore the function of SPIN1 in porcine oocyte maturation, knockdown, and overexpression techniques were used. SPIN1 mRNA was identified in maternal stages ranging from GV to MII. SPIN1 was localized in the cytoplasm and to chromosomes during meiosis. SPIN1 knockdown accelerated first polar body extrusion. Oocytes with overexpressed SPIN1 were arrested at the MI stage. SPIN1 depletion caused meiotic spindle defects and chromosome instability. The BUB3 signal was investigated, confirming that SPIN1 affects the stability of Bub3 mRNA as well as BUB3 expression. Further, overexpression of SPIN1 inhibited the degradation and regulation of G2/mitotic-specific cyclin-B1. In summation, SPIN1 regulates the meiotic cell cycle by modulating the activation of the spindle assembly checkpoint.

Keywords: BUB3; SPIN1; porcine oocyte maturation; spindle assembly checkpoint.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Anaphase*
  • Animals
  • CDC2 Protein Kinase / metabolism
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cells, Cultured
  • Chromosome Segregation
  • Female
  • Gene Expression Regulation, Developmental
  • In Vitro Oocyte Maturation Techniques
  • Metaphase*
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Oocytes / metabolism*
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Poly-ADP-Ribose Binding Proteins / genetics
  • Poly-ADP-Ribose Binding Proteins / metabolism*
  • Signal Transduction
  • Spindle Apparatus / metabolism*
  • Sus scrofa
  • Time Factors


  • Cell Cycle Proteins
  • Microtubule-Associated Proteins
  • Phosphoproteins
  • Poly-ADP-Ribose Binding Proteins
  • spindlin
  • CDC2 Protein Kinase