Rbfox1 Mediates Cell-type-Specific Splicing in Cortical Interneurons

Neuron. 2018 Nov 21;100(4):846-859.e7. doi: 10.1016/j.neuron.2018.09.026. Epub 2018 Oct 11.


Cortical interneurons display a remarkable diversity in their morphology, physiological properties, and connectivity. Elucidating the molecular determinants underlying this heterogeneity is essential for understanding interneuron development and function. We discovered that alternative splicing differentially regulates the integration of somatostatin- and parvalbumin-expressing interneurons into nascent cortical circuits through the cell-type-specific tailoring of mRNAs. Specifically, we identified a role for the activity-dependent splicing regulator Rbfox1 in the development of cortical interneuron-subtype-specific efferent connectivity. Our work demonstrates that Rbfox1 mediates largely non-overlapping alternative splicing programs within two distinct but related classes of interneurons.

Keywords: ASD; Rbfox1; alternative splicing; connectivity; interneuron; neurodevelopment; synapse.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Alternative Splicing / physiology*
  • Animals
  • Cerebral Cortex / cytology
  • Cerebral Cortex / physiology*
  • Interneurons / cytology
  • Interneurons / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Organ Culture Techniques
  • RNA Splicing Factors / physiology*


  • RNA Splicing Factors
  • Rbfox1 protein, mouse