Categorization of endoplasmic reticulum stress as accumulation of unfolded proteins or membrane lipid aberrancy using yeast Ire1 mutants

Biosci Biotechnol Biochem. 2019 Feb;83(2):326-329. doi: 10.1080/09168451.2018.1530098. Epub 2018 Oct 13.


Endoplasmic reticulum (ER)-located protein Ire1 triggers the unfolded protein response against ER-stressing stimuli, which are categorized as ER accumulation of unfolded proteins or membrane lipid-related aberrancy. Here we demonstrate that by using yeast Ire1 mutants, we can distinguish the category to which a stress-inducing stimulus belongs. For instance, ethanol was found to activate Ire1 through both types of cellular damage.

Keywords: Endoplasmic reticulum; UPR; membrane lipid; stress response; yeast.

MeSH terms

  • Basic-Leucine Zipper Transcription Factors / genetics
  • Basic-Leucine Zipper Transcription Factors / metabolism
  • Endoplasmic Reticulum Stress*
  • Lipid Metabolism*
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Membrane Lipids / metabolism*
  • Mutation*
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism*
  • Proteins / metabolism*
  • RNA, Messenger / genetics
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Unfolded Protein Response*


  • Basic-Leucine Zipper Transcription Factors
  • HAC1 protein, S cerevisiae
  • Membrane Glycoproteins
  • Membrane Lipids
  • Proteins
  • RNA, Messenger
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • IRE1 protein, S cerevisiae
  • Protein Serine-Threonine Kinases