Artesunate may inhibit liver fibrosis via the FAK/Akt/β-catenin pathway in LX-2 cells

BMC Pharmacol Toxicol. 2018 Oct 16;19(1):64. doi: 10.1186/s40360-018-0255-9.

Abstract

Background: An increasing number of studies are investigating the effects of Chinese medicine on hepatic fibrosis, but only few studies have examined the anti-fibrogenic properties of Artesunate (ART). The aim of the present study was to explore the anti-fibrotic effects of ART on LX-2 cells, the human HSC cell line, and to determine potential molecular mechanisms via the focal adhesion kinase (FAK)/ protein kinase B (Akt)/ β-catenin pathway.

Methods: LX-2 cells were stimulated with different concentration of ART (0, 12.5, 25 and 50 μg/ml) for 12, 24, 48 or 72 h, their proliferation was analyzed using the Cell Counting Kit-8 (CCK-8) assay. LX-2 cells were treated with different doses of ART (0, 12.5, 25 and 50 μg/ml) for 24 h, their apoptosis was measured using flow cytometry, the levels of mRNAs encoding collagen I or α-smooth muscle actin (α-SMA) were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and the levels of key proteins in the FAK/Akt/β-catenin signaling pathway were assessed by western blotting. Specific inhibitors of FAK were added to the LX-2 cells cultures to explore the potential signaling.

Results: Exposing LX-2 cells to ART efficiently inhibited their proliferation, significantly promoted early apoptosis in a dose-dependent manner, and markedly downregulated the mRNA expression of α-SMA and collagen I. In addition, ART, similar to FAK inhibitor PF562271 significantly inhibited the FAK/Akt/β-catenin signaling pathway by reducing the levels of phosphorylated FAK, Akt and GSK-3β.

Conclusions: Our present study shows that ART could regulate the proliferation, apoptosis and activation of LX-2. Meanwhile, the anti-fibrogenic mechanisms of ART was correlated with FAK/Akt/β-catenin pathway. Future research should verify and extend these findings, as well as explore other molecules and therefore serve as useful therapeutic targets.

Keywords: Activation; Artesunate; FAK/Akt/β-catenin; Hepatic stellate cells; Liver fibrosis.

MeSH terms

  • Actins / genetics
  • Apoptosis / drug effects
  • Artesunate / pharmacology*
  • Cell Line
  • Cell Proliferation / drug effects
  • Collagen Type I / genetics
  • Focal Adhesion Kinase 1 / metabolism*
  • Humans
  • Liver Cirrhosis / drug therapy*
  • Liver Cirrhosis / metabolism
  • Proto-Oncogene Proteins c-akt / metabolism*
  • Signal Transduction
  • beta Catenin / metabolism*

Substances

  • Actins
  • CTNNB1 protein, human
  • Collagen Type I
  • beta Catenin
  • Artesunate
  • Focal Adhesion Kinase 1
  • PTK2 protein, human
  • Proto-Oncogene Proteins c-akt