Tandemly arranged gene clusters of malarial parasites that are highly conserved and transcribed

Mol Biochem Parasitol. 1987 Jan 15;22(2-3):249-57. doi: 10.1016/0166-6851(87)90056-9.

Abstract

A molecular clone containing a 5.8 kb Eco RI fragment was isolated from a genomic library of the rodent malarial parasite Plasmodium yoelii. The P. yoelii genome contains about 150 copies of this sequence, making up almost 3% of the DNA. These sequences are tandemly arrayed in head-to-tail configurations with the unit length of the repeat being 5.8 kb. Several poly(A+) RNAs of P. yoelii ranging from 1.6 to 0.3 kb are recognized by the 5.8 kb clone. Five additional species of malarial parasites (P. chabaudi, P. berghei, P. falciparum, P. knowlesi, and P. cynomolgi) contain tandemly repeated arrays of sequences having the same unit length of 5.8 kb, which readily hybridize to the sequence cloned from P. yoelii.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cloning, Molecular
  • DNA / analysis*
  • DNA / genetics
  • DNA Restriction Enzymes
  • Deoxyribonuclease EcoRI
  • Electrophoresis, Agar Gel
  • Genes*
  • Mice
  • Nucleic Acid Hybridization
  • Plasmodium / genetics*
  • Plasmodium berghei / genetics
  • Plasmodium falciparum / genetics
  • Protein Biosynthesis
  • Repetitive Sequences, Nucleic Acid
  • Transcription, Genetic

Substances

  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonuclease EcoRI