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. 2018 Oct 18;13(10):e0205574.
doi: 10.1371/journal.pone.0205574. eCollection 2018.

Prevalence and concordance of oral and genital HPV in women positive for cervical HPV infection and in their sexual stable partners: An Italian screening study

Gianguido Cossellu  1 , Luigi Fedele  2 , Bouabid Badaoui  3 , Francesca Angiero  4 , Giampietro Farronato  1 , Ermelinda Monti  2 , Carlo Antonio Liverani  2 , Chiara Gorni  5 , Sara Botti  5
Affiliations

Prevalence and concordance of oral and genital HPV in women positive for cervical HPV infection and in their sexual stable partners: An Italian screening study

Gianguido Cossellu et al. PLoS One. .

Abstract

Objectives: This cross-sectional study aimed to evaluate the prevalence and type of oral HPV-infection in women with a cervical HPV-lesion and in the oral and genital mucosa of their male partners.

Methods: The study group comprised 44 sexually-active women, 20-45 years with abnormal PAP smear, not more than 6 months prior to referral together with the male partners cohabiting in stable partnerships. A detailed questionnaire was administered concerning the HPV-related risk factors. Oral swabs, oral rinses, cervical swabs and urine samples were collected. HPV DNA was detected using two different polymerase chain reactions (PCRs): MY09-11 and FAP59-64. Positive samples were genotyped by Sanger sequencing and the INNO-LiPA HPV Genotyping Extra II probe assay. The association with risk factors was assessed by fitting a generalized model, using the General Linear Model function in the R-software; correlations were calculated between all data.

Results: HPV was detected in 84% of Cervical Samples, in 24.3% of oral samples and in one urine sample. Only 27% of the HPV-positive results were identical with both PCR DNA assays. 8 male had oral HPV-positive samples different from women cervical samples. In one couple the urine-male sample had the same HPV present in the female-cervical sample. A significant association resulted between women/oral sex practices and men/n. of partners.

Conclusions: This study reports that women (20.4%) with a diagnosis of cervical-HPV and their male partners (30,7%) are at high risk for subclinical oral HPV infection.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Molecular work flow.
Fig 2
Fig 2. Prevalence of HPV genotype in CS samples using MY09-MY11, FAP59-FAP64, or INNO-LiPA HPV Genotyping Extra II probe assays; HPV genotype (X-axis) and percentage of HPV genotype (y-axis).
Fig 3
Fig 3. HPV genotyping: Percentage of HPV type in oral samples using MY09-MY11, FAP59-FAP64, or INNO-LiPA HPV Genotyping Extra II probe assays.
See this image and copyright information in PMC

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Grants and funding

The authors received no specific funding for this work.