Non-invasive Point-of-Care Device To Diagnose Acute Mesenteric Ischemia

ACS Sens. 2018 Nov 26;3(11):2296-2302. doi: 10.1021/acssensors.8b00558. Epub 2018 Nov 5.


Inadequate blood supply to the intestine can lead to acute mesenteric ischemia (AMI), with a mortality rate ranging from 60% to 90%. This high mortality rate is partially due to late detection and the lack of efficient early diagnostic tests. There is an urgent need for a point-of-care tool for immediate bedside diagnosis. Here we present for the first time a rapid and non-invasive electrochemical biosensor device based on non-faradic impedance spectroscopy to detect intestinal fatty-acid binding protein (I-FABP) as an indication of AMI. The electrochemical biosensors consist of gold interdigitated electrodes that were fabricated using photolithographic techniques on top of silicon dioxide substrates. The electrode surfaces were functionalized with an I-FABP capture antibody (CAnB) to entice the target protein, while gold nanoparticles (GNPs) functionalized with detection antibodies (DAnB-GNPs) were utilized as a novel mechanism to enhance the detection signal. Quantification of the I-FABP concentration in the medium depended on its attachment to CAnB and DAnB-GNPs in a sandwich manner, where the latter boosts the impedance signal through its binding to the I-FABP. This non-invasive non-faradic electric biosensor device demonstrates the potential for bench-to-bedside translation with the goal of decreasing morbidity and mortality from AMI.

Keywords: acute mesenteric ischemia; bedside monitoring; impedance-based biosensor; non-invasive urine test; point-of-care device.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Adult
  • Antibodies / immunology
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods
  • Dielectric Spectroscopy
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods
  • Electrodes
  • Fatty Acid-Binding Proteins / immunology
  • Fatty Acid-Binding Proteins / urine*
  • Female
  • Gold / chemistry
  • Humans
  • Immunoassay / instrumentation
  • Immunoassay / methods
  • Intestines / chemistry
  • Limit of Detection
  • Male
  • Mesenteric Ischemia / diagnosis*
  • Metal Nanoparticles / chemistry
  • Point-of-Care Systems
  • Young Adult


  • Antibodies
  • FABP2 protein, human
  • Fatty Acid-Binding Proteins
  • Gold