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Differences in Interleukin-8 Plasma Levels Between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status


Differences in Interleukin-8 Plasma Levels Between Diabetic Patients and Healthy Individuals Independently on Their Periodontal Status

Petra Borilova Linhartova et al. Int J Mol Sci.


Chronic periodontitis (CP) and diabetes mellitus (DM) involve several aspects of immune functions, including neutrophil activity and cytokine biology. Considering the critical function of chemokine interleukin-8 (IL-8) in the inflammatory process, the aims of this study were to determine: (i) IL-8 plasma levels; (ii) IL-8 (-251A/T, rs4073) and its receptor 2 (CXCR2, +1208C/T, rs1126579) polymorphisms, and (iii) the presence of the selected periodontal bacteria in types 1 and 2 DM patients (T1DM and T2DM) and systemically healthy controls (HC) with known periodontal status. This case⁻control study comprises of 153 unrelated individuals: 36/44 patients suffering from T1DM+CP/T2DM+CP and 32/41 from HC+CP/non-periodontitis HC. Both the clinical and biochemical parameters were monitored. The genotypes were determined using qPCR, IL-8 plasma levels were measured using an ELISA kit. Subgingival bacterial colonization was analyzed with a DNA microarray detection kit. The IL-8 plasma levels differed significantly between non-periodontitis HC and T1DM+CP/T2DM+CP patients (P < 0.01). Even in HC+CP, IL-8 concentrations were significantly lower than in T1DM+CP/T2DM+CP patients (P ≤ 0.05). No significant associations between the IL-8 plasma levels and the studied IL-8 and CXCR2 polymorphisms or the occurrence of selected periodontal bacteria (P > 0.05) were found. CP does not influence the circulating IL-8 levels. Patients with T1DM+CP/T2DM+CP had higher circulating IL-8 levels than HC+CP/non-periodontitis HC.

Keywords: chemokines; chronic periodontitis; diabetes mellitus; interleukin-8; plasma; polymorphism.

Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.


Figure 1
Figure 1
Comparison of IL-8 plasma levels in T1DM+CP (N = 36) and T2DM+CP patients (N = 44) and HC+CP (N = 41) and non-periodontitis HC (N = 32). Samples with IL-8 levels under the limit of detection (<4.40 pg/mL) were assigned a value of 4.39 pg/mL. The Mann-Whitney U-test (two-tail) was used for the calculation of significant differences.

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