Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions

Anal Biochem. 1987 Mar;161(2):559-66. doi: 10.1016/0003-2697(87)90489-1.


Most assays for superoxide dismutase depend upon competition between the enzyme and some indicating scavenger for O-2. We have investigated the effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium. Our results should help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cytochrome c Group
  • Hydrogen-Ion Concentration
  • Nitroblue Tetrazolium
  • Superoxide Dismutase / analysis*
  • Xanthine Oxidase


  • Cytochrome c Group
  • Nitroblue Tetrazolium
  • Superoxide Dismutase
  • Xanthine Oxidase