Petiveria alliacea Suppresses Airway Inflammation and Allergen-Specific Th2 Responses in Ovalbumin-Sensitized Murine Model of Asthma

Chin J Integr Med. 2018 Dec;24(12):912-919. doi: 10.1007/s11655-018-2566-5. Epub 2018 Oct 19.


Objective: To examine the effect of metanol extract of Petiveria alliacea (PM) on airway inflflammation in a murine model of chronic asthma.

Methods: Two-month-old male BALB/c mice (n=6-8/group) were sensitized on days 0 and 14 by intraperitoneal injection of 20 μg ovalbumin (OVA). On day 25, the mice received an airway challenge with OVA (3%, w/v, in phosphate buffered saline). PM was administered orally by oral gavage to mice at doses of 100, 200 and 400 mg/kg body weight once daily from days 18 to 23. Control mice were orally administered phosphate buffered saline (PBS) to induce a model of asthma. At the end of the test, respiratory reactivity was assayed, the total cell number, interleukin-4 (IL-4), IL-5, IL-13, tumor necrosis factor-alpha (TNF-α) and reactive oxygen species (ROS) in the bronchoalveolar lavage fluid (BALF) were determined and the levels of serum IgE, intercellular cell adhesion molecule 1 (ICAM-1) and eotoxin were measured. In addition, lung tissue was used to qualify the IL-4, IL-5, IL-13, TNF-α and transforming growth factor beta 1 (TGF-β1). Histologic examination was performed to observe inflammatory cellular infiltration.

Results: The administration of PM in comparison with the OVA-only treated group signifificantly attenuated the infifiltration of eosinophils and other inflflammatory cells (P<0.01). Airway resistance (RI) in the OVA-only induced group was significantly higher than that of the PBS control group (P<0.01) when methacholine was added. TNF-α, IgE, TGF-β1 and cytokine levels IL-4, IL-5, IL-13 in the BALF decreased compared to control mice (P<0.01 or P<0.05). PM treatment also inhibited the production of chemokines, eotaxin and ICAM-1 in BALF (P<0.01), which improved lung function. Histopathological examination revealed that the sensitized treated PM groups had significant lower in inflammatory scores similar to dexamethasone treatments and the untreated group.

Conclusion: Administration of PM could inhibit airway inflammation, regulate cytokines, chemokines and enhance pulmonary conditions in allergic murine model of asthma.

Keywords: Petiveria alliacea; allergy; antiinflflammation; asthma.

MeSH terms

  • Allergens / immunology*
  • Animals
  • Asthma / blood
  • Asthma / drug therapy*
  • Asthma / immunology
  • Asthma / physiopathology
  • Bronchial Hyperreactivity / complications
  • Bronchial Hyperreactivity / drug therapy
  • Bronchial Hyperreactivity / immunology
  • Bronchial Hyperreactivity / physiopathology
  • Bronchoalveolar Lavage Fluid
  • Chemokines / metabolism
  • Disease Models, Animal
  • Immunoglobulin E / blood
  • Inflammation / blood
  • Inflammation / complications
  • Inflammation / drug therapy*
  • Inflammation / immunology
  • Lung / immunology
  • Lung / pathology*
  • Lung / physiopathology
  • Male
  • Methanol
  • Mice, Inbred BALB C
  • Mucus / metabolism
  • Ovalbumin / immunology*
  • Phytolaccaceae / chemistry*
  • Phytotherapy
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Reactive Oxygen Species / metabolism
  • Th2 Cells / immunology*


  • Allergens
  • Chemokines
  • Plant Extracts
  • Reactive Oxygen Species
  • Immunoglobulin E
  • Ovalbumin
  • Methanol