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. 2018 Dec;18(4):380-384.
doi: 10.1016/j.repbio.2018.10.003. Epub 2018 Oct 19.

Glycan Mapping of Recombinant Human Follicle Stimulating Hormone by Mass Spectrometry

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Glycan Mapping of Recombinant Human Follicle Stimulating Hormone by Mass Spectrometry

Sanjay Kumar Singh et al. Reprod Biol. .

Abstract

In humans, regulation of reproductive functions are carried out mainly by glycoprotein hormones namely follicle stimulating hormone (FSH), thyroid stimulating hormone (TSH), luteinizing hormone (LH) and chorionic gonadotropin (CG). Since glycans play an important role in binding of gonadotropins with their respective receptors, it is important to identify associated glycans and their pharmacological properties not only for the disease manipulation but also for making more efficacious and safer recombinant versions. With the advancement of mass spectrometry, it is possible to identify minute quantity of associated glycans. Here, we studied the N-glycans of the FSH based on mass spectrometry and report one more complex glycan species in addition to twenty four previously reported glycans. The new glycan was a tetra antennary species that may have important role in binding of FSH with receptor with higher biological activity as well as lower clearance rate and higher half-life.

Keywords: FSH; Follicle stimulating hormone; Glycan; Mass spectrometry.

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